Dog1 antibody

SKU: orb2276582

Description

Dog1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

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Item 1 of 3

Tested Applications	IHC
Dilution range	1:100-1:200
Reactivity	Human
Application Notes	Positive Control: Appendix: An at least moderate, predominantly membranous staining should be seen in Cajal cells in the muscular wall and a weak membranous staining should be seen in columnar epithelial cells in the basal part of the crypts of the appendix. Negative Control: Appendix: Staining should be absent in the majority of cells in the muscular wall and in epithelial cells located at the surface. Cellular Localization: Cell Surface and Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b, kappa
Clone No.	Biorbyt-201M
Immunogen	Recombinant human DOG-1 protein fragment (aa 2-101) (exact sequence is proprietary)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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Anoctamin 1; Calcium Activated Chloride Channel; Discovered On Gastrointestinal Stromal Tumors Protein 1; TAOS2; ORAOV2; TMEM16A

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Dog1 Tumor periphery of a GIST showing intensive DOG1 staining in all tumor cells.

Parotid gland: Strong DOG1 staining of the apical membranes of secreting cells.

Seminal vesicle: Strong DOG1 staining of apical membranes of glandular cells.

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UniProt

Q5XX6

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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Dog1 antibody (orb2276582)

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Applications:	WB
Predicted Reactivity:	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Porcine
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF, IHC
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Dog1 antibody

Description

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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UniProt Details

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Protocol Information

Dog1 antibody (orb2276582)

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