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DYKDDDDK Tag (FLAG) Antibody Agarose Conjugated
SKU: orb344560
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IP, WB |
|---|---|
| Dilution range | IP: 10 µL resin binds >1 µg FLAG fusion protein |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | 29E4.G7 |
| Immunogen | Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH. |
| Purity | Anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody coupled to activated agarose. This product is intended for purification of proteins containing the FLAG epitope tag sequence. Binding Specificity: Anti-DYKDDDDK Affinity Gel binds the FLAG epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide is recommended for competitive elution to recover fusion protein (see protocol). |
| Conjugation | Agarose |
Storage & Handling
−| Storage | Store vial at 4°C prior to opening. |
|---|---|
| Form/Appearance | Suspension of agarose beads |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.99 mg |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-FLAG tag agarose gel, mouse anti-DYKDDDDK agarose gel, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody
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SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel. Lane 1: Cell lysate before purification. Lane 2: Flow through (used cell lysate). Lane 3: Purified DYKDDDDK (FLAG® tag) recombinant protein (arrowhead). Load: (6 µl per lane). Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.
SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel. Lane 1: crude lysate containing over-expressed DYKDDDK-tagged recombinant protein. Lane 2: unbound flow-through of consisting of endogenous E.coli proteins. Lane 3: enriched recombinant protein. Load: 5 µg protein, 15 µl flow through. Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.
SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel. Lane 1: DYKDDDDK tagged recombinant protein. Lane 2: flow through. Lane 3: wash. Lane 4: eluted fraction one. Lane 5: eluted fraction two. Lane 6: eluted fraction three. Lane 7: blank. Lane 8: molecular weight markers. Load: 5 µg protein lane 1, 15 µl per each other lane. Predicted/Observed size: 135kDa for DYKDDDDK tagged recombinant protein.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
DYKDDDDK Tag (FLAG) Antibody Agarose Conjugated (orb344560)
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