E3330

SKU: orb1300433

Description

E3330 is a potent and selective small molecule inhibitor of the APE1/Ref-1 protein. By targeting APE1's redox function, it effectively suppresses NF-κB DNA-binding activity. This inhibitor is a valuable research tool for studying oxidative stress signaling, inflammation, and cancer biology in both cellular and animal models.

Research Area

Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Immunology & Inflammation, Metabolism Research, Molecular Biology, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	136164-66-4
MW	378.46
Purity	99.52% (May vary between batches)
Formula	C₂₁H₃₀O₆
SMILES	CCCCCCCCC\C(=C/C1=C(C)C(=O)C(OC)=C(OC)C1=O)C(O)=O
Target	DNA/RNA Synthesis,HIF/HIF Prolyl-Hydroxylase,Reactive Oxygen Species,ROS,VEGFR,NF-κB
Solubility	10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.28 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:70 mg/mL (184.96 mM);DMSO:50 mg/mL (132.11 mM)

Bioactivity

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In Vivo	In mice with endotoxin-mediated hepatitis, E3330 (300 mg/kg, p.o.) attenuates the elevation of plasma tumor necrosis factor activity and protectes mice from liver injury. In Rat model, E3330 (100 mg/kg, p.o.) also protectes rats from severe liver injury induced with endotoxin plus galactosamine. Read more...
In Vitro	E3330 affects hemangioblast development in vitro via inhibition of Ape1 redox activity. E3330 inhibits the growth of human pancreatic cancer cell line PANC1, XPA1, MIAPACA, BxPC3, and PK9. E3330 also promotes exit of cell cycle in PANC1 cells, inhibits the DNA-Binding activity of HIF-1α and migration of pancreatic cancer cells. In JHH6 cells, E3330 prevents the functional activation of NF-κB via the alteration of APE1 subcellular trafficking and reduces IL-6 and IL-8 expression induced by TNF-α and FAs accumulation through blockage of the redox-mediated activation of NF-κB. Read more...
Cell Research	PANC1 cells are placed in one well of a 12-well plate and treated with 5 to 30 μM E3330. After a 24, 48, and 72 h of culture, the cells are washed with PBS and stained with trypan blue, and cell viability is examined by counting the live cell numbers.(Only for Reference) Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Activator Protein 1, anticancer, APE1, APE1(Ref-1), AP-1, APX3330, APX-3330, APX 3330, DNASynthesis, E 3330, DNA-binding, DNA/RNA Synthesis, DNA Synthesis, HIF, HIFProlylHydroxylase, HIFs, HIF-PH, HIF/HIFProlylHydroxylase, HIF Prolyl-Hydroxylase, HIF ProlylHydroxylase, HIF/HIF ProlylHydroxylase, HIF/HIF Prolyl-Hydroxylase, Hypoxia-inducible factors, HUVECs, E3330, E-3330, endonuclease, EPCs, H1975, NSCLC cells, Nuclear factor-κB, Nuclear factor-kappaB, NF-κB, orally, neuroprotective, pancreatic cancer, PANC1, PCECs, Inhibitor, inhibit, IL-8, VEGFR, Vascular endothelial growth factor receptor, Reactive Oxygen Species, REF-1, RNA Synthesis, RNASynthesis, TNF-α

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

6439397 ↗

Key Properties

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No computed properties available.

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E3330

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Quality Guarantee

Compound Identifiers

Key Properties

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Protocol Information

E3330 (orb1300433)

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