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FN1 Antibody

SKU: orb1671926

Description

FN1 Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC-Fr, SBR, WB
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
ClonalityRecombinant
IsotypeIgG kappa
Clone No.G4
Immunogen2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells.
TargetFN1
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

EDB(+) FN, Extra domain-B containing fibronectin

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FN1 Antibody

Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of G4 (orb1671926) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671926- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

FN1 Antibody (orb1671926)

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0.2 mg
$ 1,090.00
DispatchUsually dispatched within 5-10 working days
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