EDANS

SKU: orb1297714

Description

EDANS acid is a quenched fluorogenic substrate used in Resonance Energy Transfer (RET) assays for retroviral protease activity. It is widely applied in biochemical research for in vitro enzyme kinetic studies and high-throughput screening of protease inhibitors, particularly in HIV and other retroviral research.

Images & Validation

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Key Properties

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CAS Number	50402-56-7
MW	266.32
Purity	99.73% (May vary between batches)
Formula	C₁₂H₁₄N₂O₃S
SMILES	NCCNc1cccc2c(cccc12)S(O)(=O)=O
Target	Others
Solubility	10% DMSO+40% PEG300+5% Tween-80+45% Saline:1 mg/mL (3.75 mM);DMSO:13.89 mg/mL (52.16 mM)

Bioactivity

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In Vitro

EDANS is one of the most popular donors for developing FRET-based protease substrates and nucleic acid probes. EDANS is often paired with DABSYL or DABCYL in FRETbased probes. Its fluorescence is environment-sensitive.

Cell Research

1. Detection of protease activity Experimental steps: 1. Prepare EDANS substrate solution: Dissolve the EDANS-labeled substrate in an appropriate buffer (such as PBS or experimental buffer), and the concentration is usually 1-50 μM, which is adjusted according to the experimental requirements. 2. Add protease: Add a protease (such as reverse transcriptase or other protease) to the EDANS substrate solution. 3. Incubate the reaction system at the optimal temperature, usually for 30-60 minutes. 4. Fluorescence detection: Use a fluorescence spectrophotometer or fluorescence measuring instrument, the excitation wavelength is usually 340 nm and the emission wavelength is about 490 nm to detect the fluorescence signal of EDANS. 5. Data analysis: Analyze protease activity based on fluorescence intensity. The higher the fluorescence signal intensity, the stronger the protease activity. 2. FRET experiment (EDANS as donor) Experimental steps: 1. Prepare substrates with EDANS and FRET receptors (such as DABCYL): Synthesize or purchase substrates with EDANS as donor fluorescent molecules and DABCYL as acceptor fluorescent molecules. 2. Add enzyme or protease: Add enzyme or protease to the substrate solution and allow the reaction to occur. 3. Fluorescence measurement: The excitation wavelength is 340 nm, and the emission wavelength of the monitored receptor (such as the emission wavelength of DABCYL is about 460 nm). 4. Results Analysis: After substrate cleavage, the energy transfer between EDANS and DABCYL will be reduced, and fluorescence signal changes can be used to monitor enzyme activity. 3. Study on enzyme kinetics and inhibitors: Experimental steps: 1. Prepare substrate and enzyme solutions: Perform experiments in EDANS-labeled substrate and enzyme solutions at different concentrations. 2. Inhibitor research: Add different concentrations of inhibitors to study their effects on enzyme activity. 3. Incubation and fluorescence detection: Incubate the reaction according to the protease activity detection and measure the fluorescence signal, thereby calculating the enzyme kinetic parameters or inhibitory constant (IC50). Notes: 1. Buffer compatibility: Ensure that the buffer used does not quench or interfere with the fluorescent signal. Phosphate buffer (PBS) or Tris buffer is usually used. 2. Protease Inhibitor: When conducting inhibitor studies, use appropriate protease inhibitors to ensure the specificity of the experiment and avoid interference from other non-targeting enzymes. 3. Sensitivity of fluorescence measurement: Use appropriate fluorescence filters for accurate fluorescence detection. The excitation wavelength of EDANS is 340 nm and the emission wavelength is about 490 nm.

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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resonance energy transfer, retroviral protease, 1,5-EDANS, fluorogenic substrate, EDANS acid [5-((2-Aminoethyl)amino)napht, EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid], EDANS

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Compound Identifiers

PubChem CID

92329 ↗

Key Properties

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No computed properties available.

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EDANS (orb1297714)

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Purity:	98.00%
CAS Number:	100900-07-0
MW:	288.3
Formula:	C₁₂H₁₃N₂NaO₃S

EDANS

Description

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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