Mouse GM-CSF ELISA Kit

SKU: orb3145551

Description

Mouse GM-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse GM-CSF.

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications

ELISA

Application Notes

Mouse GM-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate GM-CSF in samples. An antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Key Properties

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Reactivity	Mouse
Sample Types	Cell culture supernatants#Serum#Plasma#Other biological fluids
Range	7.8 pg/mL-500 pg/mL
Sensitivity	4 pg/mL

Storage & Handling

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Storage	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Expiration Date	6 months from date of receipt.
Hazard Information	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Disclaimer	For research use only

Alternative Names

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GM-CSF; GMCSF; Colony stimulating factor; Granulocyte Macrophage Colony Stimulating Factor; CSF 2; CSF2

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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