COL18A1 Antibody (Biotin)

SKU: orb1272700

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Reactivity	Human
Application Notes	ELISA:Sandwich:To detect hEndostatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Endostatin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEndostatin. Western Blot:To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEndostatin (human Endostatin). Human Endostatin specific antibody was purified by affinity chromatography and then biotinylated.
Target	COL18A1
Conjugation	Biotin

Storage & Handling

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Storage	Endostatin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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KS, KNO, KNO1

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To detect hEndostatin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Endostatin (orb1272701) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEndostatin.

To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

COL18A1

UniProt

P39060

RefSeq:P39060

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P39060

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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