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ENOA Antibody (C-term)
SKU: orb1931370
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB19995 |
| Target | This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the C-terminal region of human ENOA. |
| Molecular Weight | 47169 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1
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ENOA Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ENOA Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
ENOA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ENOA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
ENOA Antibody (C-term) western blot analysis in Jurkat, MCF-7, U251 cell line and mouse brain tissue lysates (35 ug/lane). This demonstrates the ENOA antibody detected the ENOA protein (arrow).
Quick Database Links
Gene Symbol
This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the C-terminal region of human ENOA.
UniProt
RefSeq (Protein):NP_001419.1, NP_001188412.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001419.1, NP_001188412.1 |
|---|
Documents Download
Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ENOA Antibody (C-term) (orb1931370)
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