Enolase rabbit pAb Antibody

SKU: orb765139

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Description

Rabbit polyclonal antibody to Enolase

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human NSE. AA range:371-420
Molecular Weight	47
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-ENO2 antibody, anti-Gamma-enolase antibody, anti-2-phospho-D-glycerate hydro-lyase antibody, anti-Enolase 2 antibody, anti-Neural enolase antibody, anti-Neuron-specific enolase antibody, anti-NSE antibody

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Immunofluorescence analysis of Hela cell. 1, Enolase Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Western Blot analysis of various cells using Enolase Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of HepG2 cells using Enolase Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of A549 cells, using NSE Antibody. The picture on the right is blocked with the synthesized peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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