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ERBB3 Rabbit Polyclonal Antibody

SKU: orb10373

Description

ERBB3 Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Equine, Gallus, Mouse, Rabbit

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human HER3 (661-760/1342aa)
TargetERBB3
Molecular Weight147 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ErbB-3; FERLK; HER3; LCCS2; MDA-BF-1; VSCN1; c-erbB-3; c-erbB3; erbB3-S; p180-ErbB3; p45-sErbB3; p85-sErbB3; Erbb3r; nuc-ErbB3; ERBB3_HUMAN; ERBB3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3; 2.7.10.1; ERBB3_MOUSE; Glial growth factor receptor; ERBB3_RAT;

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ERBB3 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ErbB-3) Polyclonal Antibody, Unconjugated (orb10373) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

ERBB3 Rabbit Polyclonal Antibody

Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ErbB 3 Polyclonal Antibody, Unconjugated (orb10373) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

ERBB3 Rabbit Polyclonal Antibody (orb10373)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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