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F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed
SKU: orb348404
Description
Images & Validation
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| Tested Applications | FLISA, IF, WB |
|---|---|
| Dilution range | FLISA: 1:10,000-1:50,000, IF: 1:500-1:2500 |
| Reactivity | Golden Hamster, Hamster |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | Golden Syrian and Armenian Hamster IgG whole molecules |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian and Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Golden Syrian and Armenian Hamster IgG and Golden Syrian and Armenian Hamster Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) and Mouse or Rat Serum Proteins. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Goat F(ab')2 Anti-Hamster IgG Antibody Fluorescein Conjugation, Goat F(ab')2 Anti-Hamster IgG FITC Conjugated Antibody, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Fluorescein Conjugation, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG FITC Conjugated Antibody
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Western blot of Fluorescein conjugated Goat F(ab')2 Anti-Hamster IgG Pre-Adsorbed secondary antibody. Lane 1: Golden Syrian Hamster IgG. Lane 2: Armenian Hamster IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Hamster IgG. Other band(s): None.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed (orb348404)
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