FAK (phospho Tyr397) rabbit pAb Antibody

SKU: orb764367

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Description

Rabbit polyclonal antibody to FAK (phospho-Tyr397)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution Range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397. AA range:363-412
Molecular Weight	119
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-PTK2 antibody, anti-FAK antibody, anti-FAK1 antibody, anti-Focal adhesion kinase 1 antibody, anti-FADK 1 antibody, anti-Focal adhesion kinase-related nonkinase antibody, anti-FRNK antibody, anti-Protein phosphatase 1 regulatory subunit 71 antibody, anti-PPP1R71 antibody, anti-Protein-tyrosine kinase 2 antibody, anti-p125FAK antibody, anti-pp125FAK antibody

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FAK (phospho Tyr397) rabbit pAb Antibody

Western blot analysis of lysates from 1) 293T, 2) AD293 , 3) Hela cells, was diluted at 1:10000, 37° 1hour. antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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