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FGF-19 Recombinant Protein
Description
Research Area
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Biological activity was determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 for this effect is 100 - 150 ng/mL. |
| Target | FGF19 |
| Protein Sequence | MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK |
| Purity | Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Storage & Handling
−| Storage | The lyophilized FGF-19 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted FGF-19 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing FGF-19 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 47.6 kDa after removal of the signal peptide. The apparent molecular mass of FGF19-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgHuman FGF-19 (N-6His) Protein [orb1471725]
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23.5 KDa
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 22.3 kDa after removal of the signal peptide. The apparent molecular mass of FGF19-His is approximately 15-25 kDa due to glycosylation.
E.coli
100 μg, 10 μg, 50 μgRecombinant Human FGF19 Protein, N-His [orb2961544]
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>90% as determined by SDS-PAGE.
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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_005108.1 |
|---|
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Protocol Information
FGF-19 Recombinant Protein (orb1230913)
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