Recombinant Filaggrin Antibody

SKU: orb2636520

Description

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

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Tested Applications	WB
Dilution Range	Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Filaggrin antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG
Clone No.	FLG/3909R
Immunogen	A portion of amino acids 198-288 was used as the immunogen for the recombinant Filaggrin antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot analysis of human A431 cell lysate using recombinant Filaggrin antibody (clone FLG/3909R). Expected molecular weight: ~434 kDa (precursor); 37-45 kDa (processed).

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UniProt

P20930

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Protocol Information

Western Blot (IB, immunoblot)

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