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FIS1 Recombinant Mouse Monoclonal Antibody
SKU: orb1152053
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Description
Research Area
Apoptosis, Cell Biology, Cell Cycle, Metabolism, Mitochondrial, Mitochondrial Markers
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, ICC/IF=1:50-200 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, Kappa |
| Immunogen | KLH conjugated synthetic peptide derived from human FIS1 |
| Target | FIS1 |
| Molecular Weight | 17 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CGI 135; CGI135; FIS 1; FIS1; Fis1 homolog; FIS1, S. cerevisiae, homolog of; FIS1_HUMAN; Fission 1(mitochondrial outer membrane) homolog(S. cerevisiae); Fission 1(mitochondrial outer membrane) homolog(yeast); Fission 1(mitochondrial outer membrane) homolog; Fission 1 homolog; hFis 1hFis1; Mitochondrial fission 1 protein; Tetratricopeptide repeat domain 11; Tetratricopeptide repeat protein 11; TPR repeat protein 11; TTC 11.
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: room temperature 2h, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: MCF-7, 2: T47D, 3: MDA-MB-231, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.
Cell line: HepG2, Fixative: 4% Paraformaldehydel, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152053.
HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (FIS1) monoclonal Antibody, Unconjugated (orb1152053) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
FIS1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
FIS1 Recombinant Mouse Monoclonal Antibody (orb1152053)
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