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FIV matrix protein p15 Antibody
SKU: orb345579
Description
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| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:5,000 - 1:20,000, WB: 1:1,000 - 1:10,000 |
| Reactivity | Virus |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of FIV Matrix Protein p15. |
| Purity | This affinity purified antibody is directed against FIV Matrix Protein p15. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.22 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Feline immunodeficiency virus matrix protein p15 antibody, FIV Matrix Protein p15, Capsid protein p24, p1, Nucleocapsid protein p13, Gag polyprotein, Core polyprotein
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Western blot using Biorbyt's affinity purified anti-FIV Matrix Protein p15 to detect p15 in the culture supernatant of FIV-infected feline CrFK cells (lane 2, arrowhead). Lane 1 is an uninfected control. Virions were enriched by ultracentrifugation, lysed, resolved by electrophoresis, and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:10000 dilution.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
FIV matrix protein p15 Antibody (orb345579)
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