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Flk-1/VEGFR2 (phospho Tyr1214) rabbit pAb
SKU: orb764189
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human VEGFR2 around the phosphorylation site of Tyr1214. AA range:1180-1229 |
| Molecular Weight | 152 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-KDR antibody, anti-FLK1 antibody, anti-VEGFR2 antibody, anti-Vascular endothelial growth factor receptor 2 antibody, anti-VEGFR-2 antibody, anti-Fetal liver kinase 1 antibody, anti-FLK-1 antibody, anti-Kinase insert domain receptor antibody, anti-KDR antibody, anti-Protein-tyrosine kinase receptor flk-1 antibody, anti-CD antigen CD309 antibody
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Western Blot analysis of various cells using Phospho-Flk-1 (Y1214) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using VEGFR2 (Phospho-Tyr1214) Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr1214) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using VEGFR2 (Phospho-Tyr1214) Antibody. The lane on the right is blocked with the phospho peptide.
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Flk-1/VEGFR2 (phospho Tyr1214) rabbit pAb (orb764189)
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