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Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP)
SKU: orb1471702
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Description
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Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | CLDN18.2 |
| Reactivity | Human |
| Tag | C-Flag Tag |
| Molecular Weight | The human full length CLDN18.2 fusion protein has a MW of 54.2 kDa |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Disclaimer | For research use only |
Alternative Names
−CLDN18.2
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ELISA plates were pre-coated with 0.5 μg/per well purified human CLDN18.2-EGFP-VLP. Serial diluted Anti-CLDN18.2 monoclonal antibody (orb1173630) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-EGFP-VLP is 877.5 ng/ml.
FACS analysis of CLDN18.2 VLP A. Negative Control 1: Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP). B. Negative Control 2: Anti-GPRC5D-CAR-Expi293 cells (an irrelevant CAR) were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP). C. Positive Control: Anti-CLDN18.2-CAR-Expi293 cells were stained with biotin labeled Protein L, followed by streptavidin-APC antibody. D. Anti-CLDN18.2-CAR-Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).
Quick Database Links
Gene Symbol
CLDN18.2
UniProt
UniProt Details
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Protocol Information
Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP) (orb1471702)
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