FTH1 Rabbit Polyclonal Antibody

SKU: orb330765

Description

Rabbit polyclonal antibody to FTH1

Research Area

Epigenetics & Chromatin, Molecular Biology, Signal Transduction

Images & Validation

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Item 1 of 6

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Goat, Guinea pig, Mouse, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human FTH1
Target	FTH1
Protein Sequence	Synthetic peptide located within the following region: NVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKM
Molecular Weight	21 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti FTH antibody, anti FTHL6 antibody, anti MGC104426 antibody, anti PIG15 antibody, anti PLIF antibody, anti FHC antibody

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FTH1 Rabbit Polyclonal Antibody [orb1175394]
Applications:
ELISA, IHC, WB
Predicted Reactivity:
Mouse, Rat
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg

Quality Guarantee

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Sample Tissue: Human Ovary Tumor, Antibody dilution: 1 ug/ml.

Sample Type: Hela, Antibody dilution: 1.0 ug/ml. FTH1 is supported by BioGPS gene expression data to be expressed in HeLa.

Sample Type: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1 ug/ml, Peptide Concentration: 5 ug/ml, Lysate Quantity: 25 ug/lane/lane, Gel Concentration: 0.12.

Kidney

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-FTH1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Jurkat cell lysate.

Quick Database Links

Gene Symbol

FTH1

UniProt

P02794

RefSeq (Protein):NP_002023

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002023

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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