GABA B Receptor 2 Antibody (HRP)

SKU: orb182097

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Description

Mouse monoclonal antibody against GABA-B Receptor 2, conjugated to HRP.

Research Area

Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IF, IHC, WB
Dilution Range	WB (1:1000)
Reactivity	Human, Mouse, Rat
Application Notes	1 µg/ml of SMC-402 was sufficient for detection of GABA(B)R2 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	N81/2 (Formerly sold as S81-2)
Immunogen	Fusion protein amino acids 861-912 of rat GABA(B)R2
Target	GABA B Receptor 2
Molecular Weight	105kDa
Purification	Protein G Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GABA B receptor 2, GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABAB R2, GABABR2, GABBR 2, GABBR2, GABR2_HUMAN, Gamma aminobutyric acid (GABA) B receptor 2, Gamma aminobutyric acid B receptor 2, Gamma aminobutyric acid GABA B receptor 2, Gamma aminobutyric acid type B receptor subunit 2, Gb 2, Gb2, GPR 51, GPR51, G protein coupled receptor 51, G-protein coupled receptor 51, GPRC 3B, GPRC3B, HG 20, HG20, HRIHFB2099, Metabotropic GABA B receptor subtype 2, BcDNA:GH07312, CG6706, CT20836, D GABA[[B]]R2, D Gaba2, FLJ36928, GH07312, OTTHUMP00000021776, OTTHUMP00000063797, R2 SUBUNIT

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone N81/2. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 2 Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA B Receptor 2 protein using Mouse Anti-GABA B Receptor 2 Monoclonal Antibody, Clone N81/2. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 μg). Load: 10 μg. Block: 5% milk. Primary Antibody: Mouse Anti-GABA B Receptor 2 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.

Quick Database Links

Gene Symbol

GABA B Receptor 2

UniProt

O88871

NCBI Gene ID

83633

RefSeq (Protein):NP_113990.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_113990.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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