GABARAP Antibody

SKU: orb377268

Description

Rabbit Anti-Human GABARAP Polyclonal

Research Area

Cancer Biology, Cell Biology, Neuroscience

Images & Validation

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Tested Applications	ICC, IF, WB
Dilution Range	WB (1:1000); ICC/IF (1:100)
Reactivity	Human, Mouse
Application Notes	A 1:1000 dilution was sufficient for detection of GABARAP on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the N-terminal of human GABARAP
Target	GABARAP
Molecular Weight	14-16kDa
Purification	Peptide Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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GABARAP, Gamma-aminobutyric acid receptor-associated protein, ATG8A, GABARAP A, MM46, GBRAP_HUMAN, FLC3B, HT004

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAP Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Endomembrane System, Cytoplasm, Golgi Apparatus Membrane, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAP Antibody (D) Composite.

Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band (s): ~40kDa (Complex).

Western blot analysis of Mouse Kidney showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band (s): ~40kDa (Complex).

Quick Database Links

Gene Symbol

GABARAP

UniProt

O95166

NCBI Gene ID

11337

RefSeq (Protein):NP_009209.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_009209.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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