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Galectin 1 Antibody / LGALS1
SKU: orb385945
Description
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | ELISA: 2-4ug/ml (order BSA/azide-free format),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, lambda |
| Clone No. | GAL1/1831 |
| Immunogen | A human partial recombinant protein corresponding to amino acids 12-108 was used as the immunogen for the Galectin 1 antibody. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Western blot testing of human 1) JEG-3 and 2) K562 cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.
Western blot testing of human HeLa cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.
Western blot testing of human 1) HeLa, 2) K562 and 3) HEK293 cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.
Immunofluorescent staining of permeabilized human HeLa cells with Galectin 1 antibody (clone GAL1/1831, green) and Reddot nuclear stain (red).
IHC testing of FFPE human prostate carcinoma with Galectin 1 antibody (clone GAL1/1831). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Galectin 1 antibody (clone GAL1/1831). These results demonstrate the foremost specificity of the GAL1/1831 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Galectin 1 antibody (clone GAL1/1831) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Galectin 1 Antibody / LGALS1 (orb385945)
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