General Free Estriol (F-E3) ELISA Kit

SKU: orb1806571

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Description

General Free Estriol (F-E3) ELISA Kit

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Application Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Free Estriol(F-E3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Free Estriol(F-E3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Estriol(F-E3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	All
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	1.5H
Range	0.63-40ng/mL
Sensitivity	0.218 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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F-E3; Free Estriol

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Gene Symbol

F-E3

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