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GFP Antibody Biotin Conjugated
SKU: orb345877
Description
Images & Validation
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| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, IF: 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Other |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Purity | Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store Anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-GFP antibody biotin conjugation, biotin conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
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Immuno-Fluorescence of Biotin Mouse anti-GFP antibody. Biotin mouse anti GFP used 1:5000.
Western Blot of Peroxidase conjugated Goat anti-Rabbit IgG antibody. Lane 1: 50 ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP Antibody Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 45 min at RT. HRP Streptavidin (p/n orb348767) was used at 1:40000 in orb348637 for 30 min at 20°C. Block: 5% Blotto (p/n orb348624) 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GFP Antibody Biotin Conjugated (orb345877)
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