GFP Antibody Fluorescein Conjugated

SKU: orb345838

Description

GFP antibody (FITC)

Images & Validation

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Tested Applications	DOT, ELISA, FC, IF, WB
Dilution range	ELISA: 1:20,000 - 1:40,000, FC: 1:2000, IF: 1:500 - 1:2,500, WB: 1:10,000-1:25,000
Reactivity	Other
Application Notes	Anti-Green fluorescent protein Fluorescein conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Purity	GFP Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-GFP antibody fluorescein conjugation, FITC conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Biorbyt FITC conjugated Rabbit anti GFP (green) stains mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP.

Western Blot of GFP Antibody Fluorescein Conjugated. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein Conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.

Quick Database Links

UniProt

P42212

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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