GFP/eGFP Tag Rabbit pAb

SKU: orb1562812

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Description

Images & Validation

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Item 1 of 2

Tested Applications	IP, WB
Dilution range	WB: 1/1000-1/10000 IP: 1/20
Reactivity	Other

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	Purified recombinant EYFP.tag full length expressed in E.coli.
Target	eGFP
Molecular Weight	Calculated MW: 27 kDa; Observed MW:
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin.
Concentration	1.06 mg/ml
Disclaimer	For research use only

Alternative Names

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GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein.

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Western blot analysis of EGFP/EYFP in CHO-K1 transfected by different EGFPfusion proteins (MW:A. 30kDa, B. 38kDa, C. 50kDa) or nontransfected (D) lysates using EGFP/EYFP antibody.

Immunoprecipitation analysis of GFP/eGFP Tag in CHO-K1 lysates transfected with EGFPCyclin D2 fragment fusion protein. IP: EGFP/EYFP antibody; WB: Cyclin D2 antibody (orb1563388).

Quick Database Links

Gene Symbol

eGFP

UniProt

C8CHS1

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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