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Glucose Oxidase Antibody
SKU: orb344168
Description
Images & Validation
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| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:2,000 - 1:10,000, WB: 1:1000 - 1:5000 |
| Reactivity | Fungi |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Glucose Oxidase [Aspergillus niger] |
| Purity | Anti-Glucose Oxidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store Glucose Oxidase Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
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Biorbyt Goat anti Glucose Oxidase antibody was used to detect Glucose Oxidase under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Biorbyt Goat anti-Glucose Oxidase antibody (p/n orb2652732) at 1:5K in orb348637, ON 4°C. Primary antibody was detected with Biorbyt Dylight 649 conjugated Donkey anti-Goat at 1:10K 1.5 hr RT in orb348637.
Western Blot of Goat anti-Glucose Oxidase antibody. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Glucose Oxidase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody (p/n orb346990) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 65-70 kDa, 65-70 kDa for Glucose Oxidase. Other band(s): None.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Glucose Oxidase Antibody (orb344168)
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