Goat anti-Alpha2-antiplasmin Antibody

SKU: orb19955

Description

Goat polyclonal antibody to SERPINF2

Images & Validation

−

Item 1 of 2

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution range	ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3-5µg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration, 3-5ug/ml.WB: Approx. 70kDa band observed in Human Duodenum and Ileum lysates (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, Blood. 2004 May 15;103(10):3783-8.; PMID: 14751930). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

−

Host	Goat
Clonality	Polyclonal
Target	Alpha2-antiplasmin
Molecular Weight	54.6; 47.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

−

anti SERPINF2 antibody, anti serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2 antibody, anti A2AP antibody, anti AAP antibody, anti ALPHA-2-PI antibody, anti API antibody, anti PLI antibody, anti serine (or cysteine) proteinase inhibitor

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

orb19955 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19955 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

Alpha2-antiplasmin

NCBI Gene ID

5345

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Flow Cytometry

View Protocol

Immunofluorescence

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols