CCAR2/DBC1 (aa842-855) Antibody

SKU: orb198491

Description

Goat polyclonal antibody to CCAR2

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:64000, WB: 0.5-2 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Porcine, Rat
Application Notes	This antibody has cross reactivity with Human, Mouse, Rat, Dog, Pig, CowELISA: antibody detection limit dilution 1:64000.WB: Approx 140kDa band observed in nuclear lysates of cell lines HEK293, MCF7 and NIH3T3 (calculated MW of 103kDa according to Human NP_066997.3 and Mouse NP_666167.3). The observed molecular weight is explained by its acetylation (Zheng et al, Mol Cell Biol. 2013 Dec;33(24):4960-70. PMID: 24126058). Recommended concentration: 0.5-2µg/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	CCAR2 / DBC1 (aa842-855)
Protein Sequence	ESHNRFSATEVTNK
Molecular Weight	103
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti CCAR2 antibody, anti cell cycle and apoptosis regulator 2 antibody, anti DBC-1 antibody, anti DBC1 antibody, anti KIAA1967 antibody, anti NET35 antibody, anti p30 DBC antibody, anti p30DBC antibody, anti cell cycle and apoptosis regulator protein 2 antibody, anti cell division cycle and apoptosis regulator protein 2 antibody, anti deleted in breast cancer 1 antibody, anti p30 DBC protein antibody

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Western blot analysis of HEK293 (Lane 1) and MCF7 (Lane 2) nuclear lysates using CCAR2 antibody

Western blot analysis of NIH3T3 nuclear lysates using CCAR2 antibody

Quick Database Links

Gene Symbol

CCAR2 / DBC1 (aa842-855)

NCBI Gene ID

57805

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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