Goat anti-CDH13 / H-cadherin Antibody

SKU: orb323032

Description

Goat polyclonal antibody to CDH13

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:4000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Application Notes	WB: Approx 110kDa band observed in Human Heart lysates and 85kDa in Human Brain (Amygdala and Hippocampus) lysates (calculated MW of 78.3kDa according to NP_001248.1). The observed molecular weights correspond to the glycosylated forms. Recommended concentrat. IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	CDH13 / H-cadherin
Protein Sequence	DSDRPERSKFRLTGK
Molecular Weight	78.3; 83.4; 73.8; 50.0; 21.1; 19.7
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti CDH13 antibody, anti cadherin 13, H-cadherin (heart) antibody, anti CDHH antibody, anti P105 antibody, anti H-cadherin antibody, anti T-cad antibody, anti T-cadherin antibody, anti cadherin-13 antibody, anti heart cadherin antibody

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orb323032 (0.3µg/ml) staining of Human Heart (A), Amygdala (B) and Hippocampus (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CDH13 / H-cadherin

NCBI Gene ID

1012

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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