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ENC1 Antibody

SKU: orb12293

Description

Goat polyclonal antibody to ENC1

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Mouse, Porcine
Application Notes
WB: Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.. IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetENC1
Protein SequenceTSVSHDKLPKVQC
Molecular Weight66.1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti CCL28 antibody, anti ectodermal-neural cortex (with BTB-like domain) antibody, anti ectodermal-neural cortex 1 (with BTB-like domain) antibody, anti ectoderm-neural cortex protein 1 antibody, anti ENC1 antibody, anti ENC-1 antibody, anti FLJ39259 antibody, anti kelch-like 35 antibody, anti kelch-like 37 antibody, anti kelch-like protein 37 antibody, anti KLHL35 antibody, anti KLHL37 antibody, anti NRPB antibody, anti nuclear matrix protein NRP/B antibody, anti nuclear restricted protein, BTB domain-like (brain) antibody, anti OTTHUMP00000128235 antibody, anti OTTHUMP00000222054 antibody, anti PIG10 antibody

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ENC1 Antibody

orb12293 (1µg/ml) staining of Rat Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ENC1 Antibody

orb12293 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ENC1 Antibody (orb12293)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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