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FOXN2/HTLF Antibody
SKU: orb18547
Description
Images & Validation
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| Tested Applications | ELISA, IF |
|---|---|
| Dilution range | Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary testing showed a band at approx. 40kDa in K562, Caco-2 and U251 cell lysate after 3µg/ml antibody staining (calculated MW of 37.7kDa according to NP_002149.2). Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nuclei of Caco-2 and U251 cells. Recommended concentration: 10µg/ml. |
| Predicted Reactivity | Human |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | FOXN2 / HTLF |
| Molecular Weight | 37.7 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti forkhead box N2 antibody, anti FOXN2 antibody, anti HTLF antibody, anti human T-cell leukemia virus enhancer factor antibody, anti T-cell leukemia virus enhancer factor antibody
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Immunofluorescence analysis of paraformaldehyde fixed U251. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed Caco-2. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
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Protocol Information
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
FOXN2/HTLF Antibody (orb18547)
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