IKAROS/IKZF1 Antibody

SKU: orb20354

Description

Goat polyclonal antibody to IKZF1

Research Area

Developmental Biology, Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC
Dilution Range	ELISA: 1:1000, WB: 1-3 μg/ml, IHC-P: 2.5-3.8µg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 65kDa band observed in lysates of cell line MOLT4 (calculated MW of 57.5kDa according to NP_006051.1). Recommended concentration: 1-3 μg/ml. Similar results were obtained with orb19989, which was developed against a different part of the protein.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	IKAROS / IKZF1
Protein Sequence	DTPYDSSASYEKEN
Molecular Weight	57.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti IKZF1 antibody, anti IKAROS family zinc finger 1 (Ikaros) antibody, anti Hs.54452 antibody, anti IK1 antibody, anti IKAROS antibody, anti LYF1 antibody, anti PRO0758 antibody, anti ZNFN1A1 antibody, anti hIk-1 antibody, anti CLL-associated antigen KW-6 antibody, anti Ikaros (zinc finger protein) antibody, anti zinc finger protein, subfamily 1A, 1 (Ikaros) antibody, anti lymphoid transcription factor LyF-1 antibody

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Western blot analysis of MOLT4 lysate using IKZF1 antibody

Immunohistochemical staining of Human Tonsil using IKZF1 antibody

Quick Database Links

Gene Symbol

IKAROS / IKZF1

NCBI Gene ID

10320

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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