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NDUFS8 Antibody
SKU: orb20637
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution range | ELISA: 1:4000, WB: 0.1-0.3 μg/ml |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Canine, Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | NDUFS8 |
| Molecular Weight | 23.7 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti CI23KD antibody, anti complex I-23kD antibody, anti NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) antibody, anti NADH dehydrogenase ubiquinone Fe-S 8 antibody, anti NADH-ubiquinone oxidoreductase 23 kDa subunit antibody, anti TYKY antibody, anti NDUFS8 antibody
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NDUFS8 Antibody (Center) [orb1937375]
IHC-P, WB
Bovine, C. elegans, Monkey, Mouse
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl - Item 1 of 3
- Item 1 of 3
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0.5 µg/ml staining of Rat Heart (A) and (2 ug/ml) Rat Brain (B) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
NDUFS8 Antibody (orb20637)
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