PALS2/VAM1/MPP6 Antibody

SKU: orb18622

Description

Goat polyclonal antibody to MPP6

Research Area

Epigenetics

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Porcine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 60kDa band observed in lysates of cell line HEK293 (calculated MW of 61.1kDa according to NP_057531.2). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	PALS2 / VAM1 / MPP6
Protein Sequence	QQVLENLTELPSS
Molecular Weight	61.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti MPP6 antibody, anti VAM1 antibody, anti p55T antibody, anti VAM-1 antibody, anti membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) antibody, anti MAGUK protein p55T antibody, anti VELI-associated MAGUK 1 antibody, anti protein associated with Lin7 2 antibody, anti PALS2 antibody

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orb18622 staining (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

PALS2 / VAM1 / MPP6

NCBI Gene ID

51678

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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