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Goat Phospho MEK1 (T286) Antibody
SKU: orb1524511
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, IP |
|---|---|
| Dilution Range | WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rabbit, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Surrounding threonine 286 |
| Target | MEK1 Phospho (T286) |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CFC3; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MKK1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 µg HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified goat anti-phospho MEK1 (T286) orb1524511 was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 was used for western blot (WB) at 1 µg/ml.
Detection of mouse Phospho-MEK1 (T286) by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified goat anti-phospho MEK1 (T286) (Cat. No. orb1524511) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human Phospho-MEK1 (T286) by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-phospho MEK1 (T286) (Cat. No. orb1524511) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002746.1 |
|---|
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Protocol Information
IHC
Immunohistochemistry
IP
Immunoprecipitation
Goat Phospho MEK1 (T286) Antibody (orb1524511)
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