PIK3CA/P110alpha Antibody

SKU: orb19357

Description

Goat polyclonal antibody to PIK3CA

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC
Dilution Range	ELISA: 1:128000, WB: 1 μg/ml)., IHC-P: 5 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration, 2-3ug/ml.Immunohistochemistry data: see also publciation Abubaker J et al. Mol Cancer. 2009 Jul 28;8:51. (PMID: 19638206).WB: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	PIK3CA / P110alpha
Protein Sequence	HQWLKDKNKGEIYD
Molecular Weight	124
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti PIK3CA antibody, anti p110-alpha antibody, anti phosphoinositide-3-kinase, catalytic, alpha polypeptide antibody, anti MGC142161 antibody, anti MGC142163 antibody, anti PI3K antibody, anti PI3-kinase p110 subunit alpha antibody, anti PtdIns-3-kinase p110 antibody, anti phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha antibody, anti phosphatidylinositol 3-kinase, catalytic, alpha polypeptide antibody, anti phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform antibody

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Immunohistochemical staining of paraffin embedded Human Brain (Cerebellar Cortex) using PIK3CA antibody

Immunohistochemical staining of Human Heart using PIK3CA antibody

Quick Database Links

Gene Symbol

PIK3CA / P110alpha

NCBI Gene ID

5290

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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