RPA1/RPA70 (aa323-337) Antibody

SKU: orb137106

Description

Goat polyclonal to RPA1

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:128000, WB: 0.3-1 μg/ml
Reactivity	Human, Mouse
Application Notes	Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1). The upper band likely represents the acetylated protein. Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	RPA1 / RPA70 (aa323-337)
Protein Sequence	CKSYEDATKITVRSN
Molecular Weight	68.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	0.3-1 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti RPA1 antibody, anti replication protein A1, 70kDa antibody, anti HSSB antibody, anti MST075 antibody, anti REPA1 antibody, anti RF-A antibody, anti RP-A antibody, anti RPA70 antibody, anti MSTP075 antibody, anti RF-A protein 1 antibody, anti RP-A p70 antibody, anti replication factor A protein 1 antibody, anti replication protein A 70 kDa DNA-binding subunit antibody, anti single-stranded DNA-binding protein antibody

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Western blot analysis of HeLa lysate (35ug protein in RIPA buffer) using RPA1 antibody

Western blot analysis of NIH3T3 (Lane 1), 3T3L1 (Lane 2), Mouse Lymph Node (Lane 3) and NSO (Lane 4) lysates using RPA1 antibody

Quick Database Links

Gene Symbol

RPA1 / RPA70 (aa323-337)

NCBI Gene ID

6117

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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