SALL1 (aa29-43) Antibody

SKU: orb20344

Description

Goat polyclonal antibody to SALL1

Research Area

Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	ELISA
Dilution Range	ELISA: 1:32000, WB: 1 μg/ml
Predicted Reactivity	Human, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Blot: Preliminary experiments gave an approx 100kDa band in Human Brain (Amygdala and Substantia nigra) and Mouse Kidney lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 140kDa according to Human NP_002959.2 and Mouse NP_067365.2, and 130kDa according to Human NP_001121364.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	SALL1 (aa29-43)
Protein Sequence	EKGQPSRPTKSKDAH
Molecular Weight	140; 130
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti SALL1 antibody, anti sal-like 1 (Drosophila) antibody, anti HSAL1 antibody, anti TBS antibody, anti ZNF794 antibody, anti sal-like 1 antibody, anti spalt-like transcription factor 1 antibody

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orb20344 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb20344 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Quick Database Links

Gene Symbol

SALL1 (aa29-43)

NCBI Gene ID

6299

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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