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STAT3 (isoform 1 and 2) Antibody
SKU: orb18540
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, IF, IHC |
|---|---|
| Dilution range | ELISA: 1:8000, WB: 2 μg/ml, IHC-P: 4-6 μg/ml |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | STAT3 (isoform 1 and 2) |
| Molecular Weight | 88.0; 88.1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Concentration | 4-8 μg |
| Disclaimer | For research use only |
Alternative Names
−anti STAT3 antibody, anti signal transducer and activator of transcription 3 (acute-phase response factor) antibody, anti APRF antibody, anti DNA-binding protein APRF antibody, anti acute-phase response factor antibody, anti FLJ20882 antibody, anti MGC16063 antibody, anti HIES antibody
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
7 µg/ml staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
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Protocol Information
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
STAT3 (isoform 1 and 2) Antibody (orb18540)
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