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TFAP2D Antibody
SKU: orb20035
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution range | ELISA: 1:64000, WB: 1 μg/ml |
| Predicted Reactivity | Bovine, Canine, Equine, Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | TFAP2D |
| Molecular Weight | 49.6 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti TFAP2D antibody, anti transcription factor AP-2 delta (activating enhancer binding protein 2 delta) antibody, anti TFAP2BL1 antibody, anti AP-2 like antibody, anti OTTHUMP00000016593 antibody, anti activating enhancer binding protein 2 beta-like 1 antibody, anti transcription factor AP-2 beta (activating enhancer binding protein 2 beta)-like 1 antibody, anti transcription factor AP-2 beta (activating enhancer-binding protein 2 beta)-like 1 antibody, anti transcription factor AP-2 beta-like 1 antibody
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Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
1 µg/ml staining of NIH3T3 nuclear cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminecence.
2 µg/ml staining of Human Ovary lysate (35 µg protein in RIPA buffer). Detected by chemiluminecence.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TFAP2D Antibody (orb20035)
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