Goat IL-6 (aa138-148) Antibody

SKU: orb2899326

Description

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IF
Dilution Range	Peptide ELISA: antibody detection limit dilution 1:64000. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 5µg/ml. Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml.
Reactivity	Human

Key Properties

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Host	Goat
Immunogen	Peptide with sequence C-EQARAVQMSTK, from the internal region of the protein sequence according to NP_000591.1; NP_001358025.1; NP_001305024.1.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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B cell stimulatory factor-2, B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HGF, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, OTTHUMP00000158544, OTTHUMP00000198486, OTTHUMP00000198489, OTTHUMP00000198490

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Immunofluorescence analysis of paraformaldehyde fixed U2OS. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic, nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

RefSeq (Protein):NP_001358025.1, NP_000591.1, NP_001305024.1

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001358025.1, NP_000591.1, NP_001305024.1

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Protocol Information

Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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