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γ H2AX Rabbit Polyclonal Antibody
SKU: orb1100614
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Description
Research Area
Cancer, DNA Damage, Epigenetics, Infectious Diseases
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S139 of human H2AX |
| Target | γ-H2AX |
| Molecular Weight | 17 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−gamma H2A.X,γH2AX, Phospho-H2AX-S139
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with orb1100614 (γ-H2AX Antibody) at dilution of 1:1000. NIH/3T3 cells were treated by UV for 15-30 minutes.
Immunofluorescent analysis of HeLa cells using orb1100614 (γ-H2AX antibody) at dilution of 1:100. HeLa cells were treated by UV for 15-30 minutes at RT.
Immunohistochemistry of paraffin-embedded mouse testis tissue slide using orb1100614 (γ-H2AX Antibody) at dilution of 1:100.
Quick Database Links
Gene Symbol
γ-H2AX
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
γ H2AX Rabbit Polyclonal Antibody (orb1100614)
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