Handelin

SKU: orb1217802

Description

Handelin has anti-inflammatory activity by inhibiting NF-κB activation and pro-inflammatory cytokine productions.

Images & Validation

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Key Properties

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CAS Number	62687-22-3
MW	552.66
Purity	>98% (HPLC)
Formula	C₃₂H₄₀O₈
SMILES	C[C@]([C@]([C@@](OC1=O)([H])[C@@](C1=C)([H])CC[C@]2(O)C)([H])[C@@]32C4)(C=C3)[C@]54[C@@]([C@H]6OC(C)=O)([H])[C@]([C@](C(C)=CC7)([H])[C@]7([H])[C@](C)(O)C6)([H])OC5=O
Target	inflammation\|NF-κB
Solubility	In Vitro: DMSO : 16.67 mg/mL (30.16 mM)

Bioactivity

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In Vivo

Handelin (Compound 1; 10-20 mg/kg; oral administration; for 30 mintues; male Sprague-Dawley rats) treatment inhibits acute inflammation in carrageenan-induced paw edema model. The serum level of IL-1β is also inhibited by Handelin in a carrageenan-induced paw edema model. Animal model: Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan. Dosage: 10 mg/kg, 20 mg/kg. Administration: Oral administration; for 30 mintues. Result: Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.

In Vitro

Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment suppresses the LPS-induced (1 μg/mL) overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Handelin also suppresseS the induction of pro-inflammatory cytokines TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells. Handelin also suppresses the activation of mitogen-activated protein kinases, including ERK and JNK signaling. Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment significantly reduces the iNOS and COX-2 mRNA levels in LPS- stimulated RAW 264.7 cells. The transcriptional activity of NF-κB stimulated with LPS is also suppressed by Handelin. In addition, the LPS-stimulated upregulation of miRNA-155 expression is suppressed by Handelin. RT-PCR Cell line: RAW 264.7 cells Concentration: 10 μM, 20 μM, 40 μM. Incubation time: 5 hours. Result: iNOS and COX-2 mRNA levels were significantly reduced. The transcriptional activity of NF-κB stimulated with LPS was also suppressed and the LPS-stimulated upregulation of miRNA-155 expression was also suppressed. Western blot analysis. Cell line: RAW 264.7 cells Concentration: 10 μM, 20 μM, 40 μM. Incubation time: Result: Suppressed the LPS-induced overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Suppressed the induction of pro-inflammatory cytokines TNF-α and IL-1β, and also also suppressed the activation of mitogen-activated protein kinases, including ERK and JNK signaling in LPS-stimulated RAW 264.7 cells.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Gene Symbol

inflammation|NF-κB

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Handelin (orb1217802)

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Purity:	99.35%
CAS Number:	62687-22-3
MW:	552.66
Formula:	C₃₂H₄₀O₈

Handelin

Description

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Key Properties

Bioactivity

Storage & Handling

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