ErbB2 Antibody / HER2

SKU: orb248331

Description

This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Images & Validation

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Tested Applications	ELISA, FACS, IF
Dilution range	ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-3ug/ml
Reactivity	Human
Application Notes	Variations in protocols, secondaries and substrates may require the antibody to be titered for optimal performance.1. This ErbB2 antibody binds to the extracellular/cell surface region of the protein.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, kappa
Clone No.	HRB2/258
Immunogen	Recombinant human protein was used as the immunogen for this ErbB2 antibody.(1)
Purification	Protein G purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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SDS-PAGE Analysis of Purified, BSA-Free ErbB2 Antibody (clone HRB2/258). Confirmation of Integrity and Purity of the Antibody.

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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