HIF1A Mouse Monoclonal Antibody

SKU: orb633012

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Description

Mouse monoclonal antibody to HIF1a

Research Area

Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IF, WB
Dilution Range	WB: 1:1000-1:10000; IF: 1:50-1:200
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Immunogen	hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
Target	HIF1A
Molecular Weight	120 kDa
Purity	≥95% as determined by SDS-PAGE
Purification	protein A+G purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BHLHE78, MOP1, PASD8

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Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with orb633012 (HIF1a Antibody) at dilution of 1:5000.

Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using orb633012 (HIF1a antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG (H+L).

Quick Database Links

Gene Symbol

HIF1A

UniProt

Q16665

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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