HighYield T7 IR680LT RNA Labeling Kit (UTP-based)

HighYield T7 IR680LT RNA Labeling Kit (UTP-based) is designed to produce randomly IR680LT-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. IR680LT (a structural analog of IRDye680LT) is hydrophilic dye ideally suited for near-infrared (NiR) fluorescence spectroscopy. UTP-PEG5-IR680LT is efficiently incorporated into RNA as sub-stitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35% UTP-PEG5-IR680LT substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-PEG5-IR680LT/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting IR680LT-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35% UTP-PEG5-IR680LT substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-PEG5-IR680LT).