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Histone H1.3/Histone H1.4(Phospho-Thr17) Rabbit mAb Antibody
SKU: orb615057
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC: 1:50-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | SR38-03 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr17 of human H1.4 |
| Molecular Weight | 30 kDa |
| Purity | ProA affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 1*TBS (pH7.4), 1%rAlbumin, 40%Glycerol. Preservative: 0.05% Sodium Azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Western blot analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on CRC cell lysates using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody at 1/500 dilution. Positive control: Lane 1: Untreated CRC whole cell lysates Lane2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysates
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin
ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
Histone H1.3/Histone H1.4(Phospho-Thr17) Rabbit mAb Antibody (orb615057)
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