HLA-DRB1 Antibody (MHC II)

SKU: orb2641774

Description

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. [RefSeq]

Images & Validation

−

Item 1 of 5

Tested Applications	FACS, IF, IHC-P, WB
Dilution Range	Flow Cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b, kappa
Clone No.	MHDRb2
Immunogen	Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

IHC testing of FFPE human tonsil and HLA-DRB1 antibody (clone MHDRb2). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human histiocytoma and HLA-DRB1 antibody (clone MHDRb2). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of Ramos lysate and HLA-DRB1 antibody. Predicted molecular weight ~30 kDa.

FACS staining of Raji cells with HLA-DRB1 antibody (clone MHDRb2); Red = isotype control, Blue = HLA-DRB1 antibody.

Immunofluorescent staining of Raji cells with HLA-DRB1 antibody (green, clone MHDRb2) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P01911

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

View Protocol

Immunofluorescence

View Protocol

Find More Protocols