HP1 gamma Mouse Monoclonal Antibody

SKU: orb499789

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Description

Research Area

DNA and RNA, Methyl lysine, Methylated DNA

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	4H6
Immunogen	Recombinant human HP1 gamma Protein
Target	CBX3
Molecular Weight	21 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CBX 3; CBX3; Chromobox homolog 3; Chromobox protein homolog 3; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma; HP1 gamma homolog; HP1Hs gamma; Modifier 2; protein HP1-GAMMA; HP1g; M32; MGC118084; CBX3_HUMAN; Modifier 2 protein;

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Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HP1 gamma) Monoclonal Antibody, Unconjugated (orb499789) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: K562 (Human) Cell Lysate at 30 ug, HL60 (Human) Cell Lysate at 30 ug, MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-HP1 gamma (orb499789) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

Quick Database Links

Gene Symbol

CBX3

UniProt

Q13185

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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